Journal: Head and Neck Pathology

Article Title: Comparison of Molecular Methods and BRAF Immunohistochemistry (VE1 Clone) for the Detection of BRAF V600E Mutation in Papillary Thyroid Carcinoma: A Meta-Analysis

doi: 10.1007/s12105-020-01166-8

Figure Lengend Snippet: Direct sequencing group—methods

Article Snippet: Positive when score 1+ Open in a separate window Direct sequencing group—methods table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Study (year) Method Number of specimens Clone VE1 vendor Dilution/incubation Interpretation Note Abd Elmageed (2017) rt-PCR 130 Spring Bioscience 1:100/60 min Capper et al. method Chen (2018) Quantitative rt-PCR 40 GBI Biotechnology NA/NA Capper et al. method de Biase (2014) ASLNA-qPCR 20 Spring Bioscience NA/NA NA Jung (2015) PCR 467 Spring Bioscience 1:50/NA No or weak staining considered negative.

Techniques: Sequencing, Staining, Mass Spectrometry

Journal: Head and Neck Pathology

Article Title: Comparison of Molecular Methods and BRAF Immunohistochemistry (VE1 Clone) for the Detection of BRAF V600E Mutation in Papillary Thyroid Carcinoma: A Meta-Analysis

doi: 10.1007/s12105-020-01166-8

Figure Lengend Snippet: PCR group—methods

Article Snippet: Positive when score 1+ Open in a separate window Direct sequencing group—methods table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Study (year) Method Number of specimens Clone VE1 vendor Dilution/incubation Interpretation Note Abd Elmageed (2017) rt-PCR 130 Spring Bioscience 1:100/60 min Capper et al. method Chen (2018) Quantitative rt-PCR 40 GBI Biotechnology NA/NA Capper et al. method de Biase (2014) ASLNA-qPCR 20 Spring Bioscience NA/NA NA Jung (2015) PCR 467 Spring Bioscience 1:50/NA No or weak staining considered negative.

Techniques: Quantitative RT-PCR, Staining, Sequencing

Journal: Head and Neck Pathology

Article Title: Comparison of Molecular Methods and BRAF Immunohistochemistry (VE1 Clone) for the Detection of BRAF V600E Mutation in Papillary Thyroid Carcinoma: A Meta-Analysis

doi: 10.1007/s12105-020-01166-8

Figure Lengend Snippet: Papillary thyroid carcinoma, classic type (a) showing diffuse cytoplasmic VE1 expression with moderate intensity (b); Papillary thyroid carcinoma, invasive follicular variant (c) showing negative VE1 immunostain (d). Evaluation of VE1 immunostain in papillary thyroid carcinoma at low power (e) may be deceptive. Higher power examination confirms diffuse but weak VE1 positivity (f) in a case with confirmed BRAF V600E mutation by next generation sequencing (unpublished data). These examples utilized prediluted Roche Ventana VE1 monoclonal antibody (Indianapolis, IN, USA) on the Leica Bond Refine System (Buffalo Grove, IL, USA) at 60 min incubation time

Article Snippet: Positive when score 1+ Open in a separate window Direct sequencing group—methods table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Study (year) Method Number of specimens Clone VE1 vendor Dilution/incubation Interpretation Note Abd Elmageed (2017) rt-PCR 130 Spring Bioscience 1:100/60 min Capper et al. method Chen (2018) Quantitative rt-PCR 40 GBI Biotechnology NA/NA Capper et al. method de Biase (2014) ASLNA-qPCR 20 Spring Bioscience NA/NA NA Jung (2015) PCR 467 Spring Bioscience 1:50/NA No or weak staining considered negative.

Techniques: Expressing, Variant Assay, Mutagenesis, Next-Generation Sequencing, Incubation

Journal: BMC Complementary and Alternative Medicine

Article Title: BreastDefend enhances effect of tamoxifen in estrogen receptor-positive human breast cancer in vitro and in vivo

doi: 10.1186/s12906-017-1621-7

Figure Lengend Snippet: Combination of TAM with BD regulates expression of apoptosis and TAM resistance related proteins in human breast tumors. Animal experiments were performed as described in Fig. . Paraffin-embedded tumor tissue sections were analyzed by immunohistochemistry using antibodies against BRAF, p21, Bcl-2 and Fibronectin (FN). Representative localization and intensity of immunoreactivities against all primary antibodies are shown. Immunohistochemical quantification of BRAF, p21, Bcl-2 and FN were determined as described in Materials and Methods . Statistical analysis by ANOVA and Holm-Sidak. Different letters above bars indicate significant differences P < 0.05 . The graphical data represent mean ( white dotted line ) +/− SD, triangles represent outliers. ( n = 10)

Article Snippet: Paraffin-embedded tumor tissue sections were analyzed by immunohistochemistry using primary antibodies against B-raf or BRAF (Clone VE1, Spring Bioscience, Pleasanton, CA, USA), Bcl-2 (Clone 124, Dako, Carpinteria, CA, USA), p21 (C-19, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and fibronectin (H-300, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining

Journal: PLoS ONE

Article Title: Genetic basis of calcifying cystic odontogenic tumors

doi: 10.1371/journal.pone.0180224

Figure Lengend Snippet: Case summaries.

Article Snippet: Specimens were then probed according to using antibodies against BRAF Val600Glu (1:4000, Clone VE1, E19290, mouse monoclonal, Spring Bioscience, Pleasanton, CA, USA), beta-catenin (1:50, Clone beta-catenin-1, M3539, mouse monoclonal, Dako, Glostrup, Denmark), and hair cortex keratin (1:500, Clone AE13, sc-57012, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and finally stained with 3,3'-diaminobenzidine.

Techniques: Next-Generation Sequencing, Sequencing, Staining

Journal: PLoS ONE

Article Title: Genetic basis of calcifying cystic odontogenic tumors

doi: 10.1371/journal.pone.0180224

Figure Lengend Snippet: A , Electropherogram of a TCT>TGT substitution at c.98 in CTNNB1, resulting in a Ser33Cys missense mutation in case #4. B , Electropherogram of a GTG>GAG substitution at position c.1799 in BRAF, resulting in Val600Glu missense mutation in case #25. Guanine is indicated by a black line, cytosine is indicated by a blue line, adenine is indicated by a green line, and thymine is indicated by a red line.

Article Snippet: Specimens were then probed according to using antibodies against BRAF Val600Glu (1:4000, Clone VE1, E19290, mouse monoclonal, Spring Bioscience, Pleasanton, CA, USA), beta-catenin (1:50, Clone beta-catenin-1, M3539, mouse monoclonal, Dako, Glostrup, Denmark), and hair cortex keratin (1:500, Clone AE13, sc-57012, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and finally stained with 3,3'-diaminobenzidine.

Techniques: Mutagenesis

Journal: PLoS ONE

Article Title: Genetic basis of calcifying cystic odontogenic tumors

doi: 10.1371/journal.pone.0180224

Figure Lengend Snippet: A, and D , Representative photomicrographs of CCOT (case #6) and ameloblastoma (case #25) specimens stained with hematoxylin and eosin. B, C, E, and F , Immunostaining for ( B and E ) BRAF Val600Glu (clone name VE1) and ( C and F ) beta-catenin. Scale bars; 20 μm.

Article Snippet: Specimens were then probed according to using antibodies against BRAF Val600Glu (1:4000, Clone VE1, E19290, mouse monoclonal, Spring Bioscience, Pleasanton, CA, USA), beta-catenin (1:50, Clone beta-catenin-1, M3539, mouse monoclonal, Dako, Glostrup, Denmark), and hair cortex keratin (1:500, Clone AE13, sc-57012, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and finally stained with 3,3'-diaminobenzidine.

Techniques: Staining, Immunostaining

Journal: PLoS ONE

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay

doi: 10.1371/journal.pone.0221123

Figure Lengend Snippet: Comparison of BRAF and NRAS results by allele specific amplification, Sanger sequencing, IHC and Idylla testing.

Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Techniques: Comparison, Amplification, Sequencing, Mutagenesis

Journal: PLoS ONE

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay

doi: 10.1371/journal.pone.0221123

Figure Lengend Snippet: A. Control of samples found positive for BRAF mutation by ASA/Sequencing but negative by IHC. B. Control of samples found negative for BRAF mutation by ASA/sequencing and positive by IHC (wrong chromogen used). C. Control of samples found positive for NRAS mutation by sequencing but negative by Idylla. Yellow dots correspond to wild type DNA copies ( BRAF , panels A and B; NRAS , panel C). Green dots correspond to mutated DNA copies ( BRAF V600E, panels A and B; NRAS Q61R, panel C). Grey dots correspond to empty wells.

Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Techniques: Control, Mutagenesis, Sequencing

Journal: PLoS ONE

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay

doi: 10.1371/journal.pone.0221123

Figure Lengend Snippet: Discordant genotyping results.

Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Techniques: Digital PCR

Journal: Diagnostics

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

doi: 10.3390/diagnostics12030751

Figure Lengend Snippet: Analysis of discordant results.

Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Techniques: Diagnostic Assay, Amplification

Journal: Diagnostics

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

doi: 10.3390/diagnostics12030751

Figure Lengend Snippet: Example of discordant results. ( a – d ), a micro-metastatic sentinel lymph node that expressed BRAF V600E when analyzed by IHC and was negative with the BRAF Idylla test despite delayed amplification. ( e – g ), a subcutaneous melanoma metastasis considered negative for IHC BRAF V600E when compared to the control. ( g ) (some background noise due to intracytoplasmic pigment), when stained with a red chromogen the interpretation remains equivocal ( h , i ) and positive with the Idylla method. ( a , e ) HES stain ×20; ( b ) Melan A IHC ×20 (Clone A103, Roche Ventana); ( c , f ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana); ( g ) BRAF V600E IHC ×40 external control showing viable tumor cells and melanophages; ( h ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana—Red detection kit); ( i ) BRAF V600E IHC ×40 external control viable tumor cells with red chromogen; ( d , j ) Amplification curves with the Idylla Explore tool.

Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Techniques: Amplification, Control, Staining

Journal: Diagnostics

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

doi: 10.3390/diagnostics12030751

Figure Lengend Snippet: Overview of studies combining immunohistochemistry and Idylla for BRAF evaluation.

Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Techniques: Immunohistochemistry, Mutagenesis, Immunostaining

Journal: Diagnostics

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

doi: 10.3390/diagnostics12030751

Figure Lengend Snippet: Comparison of Idylla and IHC for the detection of the BRAF V600E mutation in melanomas.

Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Techniques: Comparison, Mutagenesis, Expressing, Blocking Assay, Selection